FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17646943
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT42400.017.1200244
TATACTG61600.015.3577225
CTAGACT47250.015.2368064
GTATAGG32950.015.1700121
TACACTG80100.015.0790475
GTATTAG40150.014.9691751
TATACAG68700.014.8098195
GTATAAG43300.014.7047611
TCTATAC46650.014.4124293
TAGACAG87750.014.23909955
CTACACT55750.013.9809534
TACACAG111550.013.9746875
TACACCG18800.013.6088365
GTGTAGA71850.013.5824821
GTGTATA61100.013.4400211
CTTACAC67250.013.3596493
GTATAGA46150.013.2809031
GTATTAC45100.013.0623321
CTAAGAC54900.013.0052673
TATACTA45300.012.871775
Produced by FastQC (version 0.11.5)