FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17646943
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN197900.11214406937224199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG26550.017.0550691
GTACGAC17500.016.681083
CTATACT44800.016.0907844
GTATAAG45250.016.0637021
CTAGACT52100.015.8945014
GTATTAG48850.015.3677541
TACGACA24950.015.0431864
GTCTAGG50150.014.8505831
TAGACAG81600.014.7474685
TATACTG64150.014.6729245
TTATACT88450.014.4813974
TGTACGA23200.014.3810592
GTCCTAG55300.014.3294951
TCTATAC43900.014.1135063
CTAGACA67400.013.9658384
GTATTAA77250.013.959971
TCTAGAC56900.013.8206283
CTAATAC47150.013.7724413
ATAATAC73900.013.6241173
ATTATAC70600.013.4170973
Produced by FastQC (version 0.11.5)