FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17633586
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT42850.018.8850234
TATACTG63150.018.2792265
GTGTACG25300.016.9339641
GTACGAC18650.015.6331583
TATACAG68100.015.5525765
TCTATAC45150.015.2872613
TTATACT89100.015.2260264
GTATAAG44900.014.9754111
CTAATAC50550.014.8312853
GTATTAT71100.014.8132491
TATACTA49050.014.799615
CCTATAC33600.014.69833853
CTACACT57000.014.4056764
TAATACT78600.014.231964
TAGACTG64400.014.228675
TACACCG16450.014.106795
ATAATAC75300.014.0654773
TAGACAG85950.013.8456565
GTATTAG47500.013.6546181
TATATAC89200.013.4078963
Produced by FastQC (version 0.11.5)