FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29853616
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN319730.10709925390612648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT95800.017.4075374
TATACTG132150.017.2614175
CCTATAC61850.016.0813163
GTATTAG90650.015.7686351
GTATATA182300.015.2247411
CCTAGAC94350.014.6450523
TCTATAC95650.014.6328093
GTCCTAG102100.014.5836071
TACACTG163950.014.530935
TAGACTG126100.014.4527735
GTATAAG85600.014.3332311
GTATAGG62450.014.1149681
GTATAGA96400.014.0866751
CTAGACT97800.014.0675344
CTACACT114050.013.682044
TTATACT173800.013.5703254
GTATTAT156000.013.4772341
TATACAG137200.013.4571295
GTGTAGG139000.013.2401941
CTTACAC130000.013.1487173
Produced by FastQC (version 0.11.5)