FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29978415
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG137950.016.3205645
CTACACT111650.015.0975784
GTATTAG87600.015.0953441
CTATACT93300.015.0664174
TATACCG15900.014.9840075
GTCCTAG102950.014.5803621
GTATATA184400.014.5360271
GTATAGG62300.014.5328131
TACACTG159300.014.5071145
GTATAAG92800.014.3778561
CTAGACT99150.014.2976234
TAATACT161350.013.9172034
CTAATAC96050.013.8908953
GTATTAT150550.013.7686891
TATACAG153200.013.5684975
ATAATAC154250.013.5538153
GTATAGA101700.013.2367571
TAGACTG123850.013.0328185
CTAGGAC108800.013.0294983
TCTATAC93900.012.9402953
Produced by FastQC (version 0.11.5)