FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19409590
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN223410.11510289501220788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG25600.019.0832061
GTATAGG35700.017.8563631
GTATAAG49350.017.142681
TATACCG10000.016.6798785
GTATATA97850.016.6218471
GTCCTAG60300.016.400921
GTACGAC18550.016.3783473
TAGACAG88150.016.3541475
CTAGACT58300.015.1230834
TAGACTG77400.014.7773015
CTATACT50700.014.6875464
TACACTG95100.014.5952065
CCTATAC41350.014.2627573
TATACAG76050.013.942985
TGTACGA24400.013.9172172
GTATAGA56300.013.8624621
TAATACT93500.013.6985324
CCTAGAC57850.013.6959473
GTATTAT85950.013.6551911
CTATACG9750.013.4420414
Produced by FastQC (version 0.11.5)