FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19359405
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT48500.017.911824
TATACTG78450.016.9137745
TATACCG9700.015.948885
TCTATAC52850.015.7620113
TATACAG77050.015.3677055
TACACTG93800.015.097425
CTAGACT58100.014.5425584
CTACACT64500.014.4833344
ATAGGAC54100.014.4079643
TAGACAG94300.014.3232885
TAGACTG76550.014.2244175
GTATAAT77050.014.132441
GTATATA99250.013.9689611
GTATTAG52050.013.7182651
TTACACT93700.013.7164824
TATAGTG51950.013.2862075
GTATAAG54450.013.2228841
TAGGACA79050.013.0971244
GTCTAGG55000.013.0906541
GTATAGG36400.013.0775781
Produced by FastQC (version 0.11.5)