FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19359405
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN215850.11149619526013325No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG9200.022.0114525
TATACTG80600.018.1784845
CTATACT52050.017.9653854
TCTATAC57600.016.5445563
GTATAGA55150.016.4157711
GTGTACG28000.015.9538851
TATACAG79650.015.1798645
CTAGACT58350.015.0049324
GTCCTAG61450.014.7327871
TACACTG96650.014.7283335
GTATAAG50350.014.5501971
CTACACT65550.014.5380074
TACACCG18750.014.2944965
GTGTATA78400.014.2825241
GTATATA102700.014.2088471
GTATATG71450.014.0879471
CCTAGAC56000.014.0392363
GTATTAC50400.013.9448771
GTATTAG50750.013.8487061
TTATACT103250.013.7868624
Produced by FastQC (version 0.11.5)