FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19505782
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT54650.018.637434
GTGTACG26400.018.5012551
GTATAGG38200.016.3725831
CTAGACT64200.016.0505964
TCTATAC58100.015.890433
TATACTG81400.014.9271525
CTACACT69050.014.923224
TACACTG96700.014.5979895
GTATTAG48250.014.5671961
GTATATA102900.014.4715781
TACACCG19800.014.4393375
GTCCTAG66300.014.1949821
GTATAGT44600.014.1567081
CCTATAC41800.013.9646453
GTATTAA87600.013.4633261
GTATTAC52200.013.3507791
CCTAGAC61600.013.150383
TATACTA55350.013.1284485
GTGTTAG66250.013.1267811
TCTAGAC62950.013.0576043
Produced by FastQC (version 0.11.5)