FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24522257
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN277580.11319512718588667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG113950.018.035975
CTATACT77100.017.771254
TCTATAC81300.015.4608013
TACACTG133700.015.2380525
GTATATA152250.015.0654361
TATACAG114800.014.7889635
CTACACT100550.014.6931244
GTATTAG72450.014.6372641
TAATACT132800.014.1304834
ATAATAC122700.014.0311533
GTATAAG69050.013.9775041
GTACGAC27700.013.7638623
GTATTAA124300.013.6600411
GTCCTAG82950.013.6463181
TAGACTG102000.013.49107555
GTATTAC72450.013.4037871
GTATTAT121450.013.3919371
TTATACT137100.013.2527774
CCTAGAC74750.013.2293043
CTAGACT80550.013.1643364
Produced by FastQC (version 0.11.5)