FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24471022
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG109700.016.9772115
TACACTG131400.016.3470925
CTATACT72600.016.2277094
GTATAGG49550.015.9719181
CTACACT94400.015.0644954
TATACAG113700.014.9669815
GTATATA148450.014.9111741
GTATTAG73300.014.6934471
GTATAGA75500.014.6593611
TTATACT136750.014.0976374
TAGACTG104150.013.7684945
CCTACAC102150.013.5138243
TATACTA76350.013.4823545
CTAGGAC91850.013.4097243
CTAGACT82700.013.3824644
TAATACT133150.013.3169194
ATAATAC117750.013.2394233
TACACAG174800.013.139375
GTATTAC70650.013.0547511
TCTATAC76750.013.0244553
Produced by FastQC (version 0.11.5)