FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11084944
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG23050.017.7483791
GTATTAG36250.016.344551
TATACAG49400.016.1724435
TATACTG44750.015.8576045
CCTATAC23650.015.6992543
CTATACT35600.015.4450594
GTATACG7600.014.8493691
TAGACTG42500.014.3750085
TACACTG57350.014.2583575
CTACACT43900.014.130684
CTAGGAC34000.013.8230593
GTCTTAG30900.013.696021
GTGTAGA49000.013.531111
GTGTAGG42750.013.5294261
GTGTAAG42000.013.4351441
TTATACT52600.013.3123344
TAGACAG55100.013.3053625
CTAATAC36800.013.2821563
ATACTGT66600.013.0542286
GTGTTAG43450.012.9867911
Produced by FastQC (version 0.11.5)