FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11084944
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG42300.019.99643594
TGGTCGC60450.017.87936294
CGCCGTA18250.017.2517294
TATACTG42600.016.9863555
GGTCGCC53650.015.67847494
TATACCG7200.015.6627445
CTAGTAC16450.015.4366943
TCGCCGT31450.015.38994494
TACACTG58600.014.9143525
TATACAG50000.014.7543045
CTATACT36000.014.3564774
GTATAGG25950.014.3206711
GTCCTAG31750.014.2233261
TAGACTG43700.013.655625
GTATAAG34500.013.634981
CCTATAC24200.013.60223
CTACACT42600.013.5660444
CTAATAC32700.013.3740113
GTACTAG17250.013.3622811
GTCTAGG34850.013.2280831
Produced by FastQC (version 0.11.5)