FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17605382
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG79050.017.2410165
TACACTG86700.016.8580785
GTATAGA50700.016.439841
GTATAGG35600.016.402271
CTATACT54800.015.8657434
CTACACT62350.015.4520684
GTGTATA72300.014.85008051
TATACAG71200.014.4554255
GTATAAG48150.014.2787061
GTATTAG48100.014.1956471
GTCTAGA51500.013.9899611
TAGACTG67450.013.9352515
GTGTTAG59200.013.8407561
GTCCTAG51200.013.7960131
TCTATAC51050.013.7170193
CCTATAC37450.013.678673
GCTACAC49750.013.6031233
TTATACT88900.013.586284
GTATATA105500.013.5691741
CTAGGAC50500.013.4010973
Produced by FastQC (version 0.11.5)