FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17605382
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN276370.1569804051965473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG65650.019.69728394
CGCCGTA27250.018.63655594
TCGCCGT46150.018.44230594
TATACTG74550.017.0400335
TGGTCGC95250.017.03187494
CTACACT63550.016.7452834
TACACTG85500.016.2283525
GTATAGG37200.016.0538441
GGTCGCC84300.015.56270194
GTATTAG48600.015.0940791
GTCCTAG51450.014.98914051
GTATAGA53600.014.38789751
GCCGTAT26050.014.26026994
GTGTACG19800.014.2496541
GTCTAGG54050.014.0941081
GTGTATA77850.014.0739371
GTGTTAG61150.014.0725551
CCTACAC59350.014.0682543
CTAGTAC27900.013.9545163
GTGTAGG65500.013.9276781
Produced by FastQC (version 0.11.5)