FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10000.08.09474692-93
CCGAGTT7652.9907658E-47.4327873
CCCCGTT8206.627814E-46.93424651
CGTCGTG4909.230229E-56.80230792-93
AGATCGG11051.8189894E-126.679188392-93
GCGTCGT3950.0032399866.63009792-93
ATCGGAA11500.06.623025494-95
CCCAAAT32750.06.3660971
CCCATAA14652.277904E-66.1453671
AGTCGGT4650.0027408586.11613110-11
CCCGAGT9550.00369906735.95401241
CCTAATT15152.5461572E-55.62978361
CCTTAGT15453.4817742E-55.52046781
CCCTAAT11450.00461313975.37984471
TCGGAAG16251.0913936E-115.272947394-95
CCCAAAG50600.05.1504371
Produced by FastQC (version 0.11.5)