FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT4251.5250407E-68.415668592-93
CCCCGAT6250.00259613487.5790841
CGTCGTG5203.3737488E-67.33673792-93
AGCGTCG5953.4071163E-66.8133790-91
GATCGGA9651.10958354E-106.665282294-95
TCGTGTA6458.149617E-55.909385794-95
CCATAAT17702.6185626E-75.88770681
ATCGGAA10151.5790647E-85.867539494-95
AGATCGG11409.586074E-105.851069594-95
ACTTGCG5350.00203612055.764621342-43
CCACGAT11100.0032095215.5477531
CCCAAAG51100.05.2838531
CCCTAAC13550.00107947415.2438311
GTCGTGT6000.0075015835.16147994-95
CCAAAGT39500.05.0367332
Produced by FastQC (version 0.11.5)