FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA9700.08.83361494-95
ATCGGAA11000.08.00602194-95
CCCTAGA18252.382876E-106.7518391
CCCTAAG15501.9053914E-86.7267081
AGATCGG12650.06.58792792-93
TAGATCG4150.0054217496.31125792-93
CCCTAGT11751.5617062E-46.0501341
AGCGTCG6000.00125804245.55372694-95
CCCAATT20501.329081E-75.5484161
GGATTAC20300.05.371867712-13
CCCATAT16257.7288125E-55.2496551
CCCAAAG51550.05.14838461
CCTAAAT20654.9738064E-65.0491031
Produced by FastQC (version 0.11.5)