FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA10000.07.599801592-93
ATCGGAA10400.07.53586194-95
TACCGAG5700.0092314757.51841261
AGATCGG11650.07.135007492-93
CCCTAAA20857.166818E-106.16617971
CCCAAAG50600.05.92855071
CCTAGTT11406.5251766E-45.84765431
CCTTAAT18706.859427E-75.60195451
CCCGGGC17901.8159681E-65.5863071
CGAAGAA10350.0085421275.5207671
CCCTAGT11250.00353705145.5023641
CCCAAAC29208.731149E-115.38132951
TCGGAAG15905.456968E-125.377218294-95
CCATGAT25953.954483E-95.3213171
Produced by FastQC (version 0.11.5)