FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT4503.8096732E-78.47723490-91
CGTCGTG5753.0144292E-87.878694592-93
GGACGAT6655.5331894E-47.83253531
CCCTATT12054.634676E-66.68025731
AGCGTCG6257.3595693E-66.485083690-91
CGTGTAG6308.371997E-66.431469494-95
ATCGGAA11351.3460522E-106.089812894-95
TCGTGTA6253.354062E-45.72022494-95
GATCGGA9953.8174585E-75.509428594-95
CCCTAGT10350.0090837685.4899981
CCCTAGC12955.914908E-45.4846991
GTCGTGT6900.00126050545.18335292-93
CCCTAGA18752.642374E-55.0507981
Produced by FastQC (version 0.11.5)