FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8403.274181E-117.351279392-93
AGATCGG10501.364242E-106.333410392-93
TGCCGAT5350.00200995085.770881714-15
CCCTAAC11850.00107481665.6229181
CCCTTTA23007.3396222E-95.58711961
ATCGGAA11558.520146E-95.551911494-95
CCCTATC11650.00536828865.31091641
TCGGAAG14901.6552804E-105.26003294-95
TGTCCGC6350.00264107325.23608866-67
CCCTAGC13100.00379218675.086381
Produced by FastQC (version 0.11.5)