FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8500.08.13308992-93
GCGTCGT3750.00185212976.991970590-91
CCCAAAT32100.06.3425321
ATCGGAA10355.875336E-106.21870492-93
CCTAAGG16454.2027023E-76.0443921
AGATCGG10555.9044396E-95.8743490-91
CCTAATA11650.0057052685.2834391
CCCTAGA16459.512558E-55.18090771
CCCTACT17655.2671043E-55.0969241
CCCAAGT28852.235538E-95.0876261
AGAGCGC6550.00384002095.08143974-75
Produced by FastQC (version 0.11.5)