FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17701813
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA83800.011.384644594
TAGATCG39050.010.71115894
AGATCGG106250.010.39456794
ATCGGAA81750.010.0029794
TCGGAAG104750.08.97311994
CGGAAGA118250.06.478193894
CAGATCG61250.06.138345294
AAGATCG49800.05.473512694
Produced by FastQC (version 0.11.5)