FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23009186
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA92250.010.39301194
AGATCGG116800.09.29494494
ATCGGAA88150.09.2236294
TAGATCG44200.08.7190594
TCGGAAG121950.07.399409394
TATGCCG21350.06.05368148-49
CGTATGC20850.05.860752646-47
CGGAAGA138400.05.738894594
CCGTATC28700.05.649746438-39
CTCGTAT22300.05.37441744-45
TCGTATG20800.05.309952746-47
GACGCGA17354.2026317E-55.167521
Produced by FastQC (version 0.11.5)