FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23368234
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA92300.010.64237594
ATCGGAA90550.010.53662794
TAGATCG39800.09.32906294
AGATCGG115800.08.68558894
TCCGATT33900.08.6021991
GCGTCGT34150.08.11998694
CCGATTT34900.08.0801572
TCGGAAG117500.07.559932794
CGCCGTA15550.06.498347846-47
AGAGCGT71950.06.140320394
CGGAAGA137350.05.81720394
AAGATCG60200.05.074705694
Produced by FastQC (version 0.11.5)