FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22996713
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA103400.010.09083294
ATCGGAA98800.09.60923894
AGATCGG129450.08.67741594
TAGATCG43450.07.788202392-93
TCGGAAG123300.06.556316494
CGCCGTA16300.06.19933946-47
GCGTCGT38150.05.91345492-93
AAGAGCG105650.05.60525794
CGGAAGA144550.05.592485494
CGTCGTG42900.05.5873794
GCCGTAT18400.05.364089548-49
AGCGTCG49300.05.338701794
CGTATCA16555.456968E-125.25373650-51
Produced by FastQC (version 0.11.5)