FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25367296
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28250.014.14130746-47
CTCGTAT28450.013.54656644-45
AACGATC29400.013.42836638-39
TCGTATG26600.012.986835544-45
TATGCCG31000.012.96251448-49
ATCGGAA131800.010.5548194
GATCGGA140700.010.18778794
AGATCGG164050.010.11282694
AGTCGGT42900.09.4213227
AAACGAT39900.08.95210436-37
TAGATCG55300.08.62614492-93
TCTCGTA39950.08.41176942-43
GAAACGA52000.08.17955336-37
TCGGAAG163750.08.03620194
CGTGAAA51750.07.17461432-33
ATCTCGT58750.07.040003342-43
ACGATCT51050.06.950922538-39
TGAAACG51350.06.818678434-35
AGTCACG60850.06.758198728-29
CGGAAGA174550.06.65045294
Produced by FastQC (version 0.11.5)