FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23652843
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA152450.010.759521594
ATCGGAA140150.010.69775294
AGATCGG181000.08.93253794
TCGGAAG163300.08.63435894
TAGATCG53050.08.15074794
AGCGTCG59550.08.1292594
GCGTCGT43750.08.05708994
AGAGCGT90200.07.50327694
AAGATCG83150.07.12202194
AAGAGCG115450.06.879988794
CGGAAGA181200.06.562318394
GTCGTGT50000.06.391957394
TCGTGTA37550.06.383445794
CAGATCG84850.05.5391592-93
CGTCGTG52150.05.227194392-93
Produced by FastQC (version 0.11.5)