FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23484426
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA186900.09.88425394
AGATCGG220100.09.26894494
AGAGCGT110350.09.1585594
ATCGGAA173850.09.03092894
CGCCGTA21000.08.95219746-47
CGTATCA20600.08.66975150-51
TAGATCG68850.08.46600194
GCCGTAT23000.08.07159948-49
CGGAAGA205400.07.87260794
TCGGAAG197050.07.395130692-93
GCGTCGT59800.07.389026694
AAGATCG96300.07.077865694
AAGAGCG137250.06.98680194
CGTCGTG62300.06.715255794
AGCGTCG71700.06.62159894
TCGCCGT32150.06.43221646-47
GAGCGTC115250.06.403527794
TCGTGTA51100.06.255306294
GTCGTGT60450.06.220915394
CCGTATC31700.05.782233748-49
Produced by FastQC (version 0.11.5)