FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22826606
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA180200.010.22371294
ATCGGAA169250.09.66335394
AGATCGG213250.09.30038194
TAGATCG66600.08.96203894
CGTATGC20950.08.63698444-45
TCGGAAG191300.08.37754394
CTCGTAT23950.07.7514742-43
TATGCCG24450.07.400475546-47
TCGTATG22400.06.819000244-45
TATCTCG24900.06.606344740-41
CGGAAGA198500.06.582055694
AAGATCG91400.06.427485594
CAGATCG93600.05.72408894
ACACGTC76000.05.31816994
GAGATCG105050.05.0330992-93
Produced by FastQC (version 0.11.5)