FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22826606
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA179500.010.1334494
ATCGGAA168550.09.75999794
CGCCGTA19000.09.3998846-47
AGATCGG214600.09.242578594
TAGATCG64800.08.70395694
AGAGCGT104050.08.58266194
TCGGAAG188850.08.51176394
AAGAGCG134600.08.13619394
CGTATCA20150.08.04702750-51
GCCGTAT21600.07.83321648-49
CCGTATC28100.07.192063348-49
AGCGTCG71600.06.892657894
CGGAAGA196000.06.882342394
GTCGTGT59650.06.7763994
GCGTCGT56200.06.48149792-93
AAGATCG87550.06.22747292-93
TCGTGTA47450.06.141378494
GAGCGTC110500.06.12506494
GAGATCG110050.06.064691594
CGTCGTG63750.05.750754494
Produced by FastQC (version 0.11.5)