FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24479735
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA21300.011.69463246-47
CGTATCA23850.011.23250850-51
GATCGGA153400.010.66261494
GCCGTAT24350.010.42281648-49
ATCGGAA142650.09.818706594
AGATCGG186700.09.51606294
TAGATCG63000.09.02723292-93
CCGTATC31850.08.55872148-49
AGAGCGT100700.07.934676694
TCGGAAG166600.07.8147494
AGCGTCG64700.07.337141594
CGGAAGA183850.07.107075794
TCGCCGT36000.07.04987746-47
GCGTCGT49000.07.002232694
GTCGCCG48900.06.29539144-45
AAGATCG77300.06.08037494
AAGAGCG123150.05.953878492-93
GAGCGTC108900.05.826606894
TCGTGTA44100.05.648444714-15
CAGATCG88050.05.498163794
Produced by FastQC (version 0.11.5)