FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24590657
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA22200.012.17310246-47
CGTATCA23300.010.5898550-51
GATCGGA153700.010.51932294
ATCGGAA141250.010.31517194
GCCGTAT23300.010.18642748-49
AGATCGG186000.09.80442194
TAGATCG63100.08.6403694
CCGTATC32800.08.23911248-49
GCGTCGT52050.08.12690194
TCGGAAG168800.07.824148794
AGAGCGT103850.06.788720694
TCGCCGT38200.06.397737546-47
AAGATCG79700.06.13307494
CGGAAGA179100.06.088293694
AGCGTCG68250.06.06014894
TCGTGTA45550.06.036064614-15
CAGATCG90800.05.95271794
GAGCGTC112600.05.843766794
TGGTCGC50300.05.83980642-43
GTCGCCG54450.05.78313544-45
Produced by FastQC (version 0.11.5)