FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21808520
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA114550.010.66730694
AGATCGG146000.09.85020194
GATCGGA119750.09.536900594
TAGATCG47600.07.997514792-93
CGTATGC18200.07.488642746-47
CGGAAGA155650.06.97492294
TCGGAAG141850.06.593268492-93
CTCGTAT19350.06.072059644-45
TATGCCG21700.05.847638648-49
AACGATC22250.05.703149338-39
AAGATCG67700.05.657778392-93
AGTCGGT31909.094947E-125.30383837
Produced by FastQC (version 0.11.5)