FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24806857
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA172150.010.45608594
ATCGGAA161850.010.39555594
AGATCGG211200.010.34752394
TAGATCG71250.09.49849994
TCGGAAG185350.08.34220194
CGGAAGA201750.07.52430494
CAGATCG81550.06.22410894
GAGATCG123550.05.820040794
AAGATCG89050.05.75267694
Produced by FastQC (version 0.11.5)