FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24911697
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA164850.09.80781894
GATCGGA175700.09.57666394
AGATCGG212850.09.38465394
TCCGATC24600.08.9927411
TAGATCG68300.08.80830294
TCGGAAG187650.08.74137694
GCGTCGT51950.08.32348594
AGCGTCG67850.08.03546694
CGGAAGA201050.07.57432494
AGAGCGT104000.07.09527594
AAGAGCG134100.06.90462994
GAGCGTC112900.06.494318594
CGTCGTG59400.06.488291394
AAGATCG87800.06.370234594
TCGTGTA45850.05.94554494
GTCGTGT55200.05.875068794
GAGATCG128150.05.464753694
CGATCTA14951.4157737E-45.34454443
CCGATCT41550.05.2034932
Produced by FastQC (version 0.11.5)