FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23580262
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21450.010.8454744-45
GATCGGA160150.010.41772494
AGATCGG189100.010.01578494
ATCGGAA149850.09.659739594
CTCGTAT23800.09.577188542-43
TATGCCG24350.09.45729146-47
TCGGAAG172900.08.58941494
TAGATCG62900.08.218904592-93
TCGTATG21600.07.506519342-43
CGGAAGA190250.06.96619994
AAGATCG84400.05.846806592-93
CAGATCG86550.05.430056694
TCTCGTA36050.05.149505640-41
ATCTCGT50450.05.030443740-41
Produced by FastQC (version 0.11.5)