FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005405979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005405979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23828292
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA160200.010.21119694
ATCGGAA148050.09.81092594
AGATCGG194450.09.28288994
TAGATCG60350.08.996304592-93
TCGGAAG175300.08.12494894
AGCGTCG68850.07.919794694
CGTCGTG57750.07.325721394
GCGTCGT51800.07.168977394
AGAGCGT105850.07.06099994
GAGCGTC108750.06.52690994
CGGAAGA187450.06.34446594
TCGTGTA45200.06.23982994
AAGAGCG128750.05.823349592-93
AAGATCG85950.05.52376794
GTCGTGT54750.05.408991394
Produced by FastQC (version 0.11.5)