FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32559285
Sequences flagged as poor quality0
Sequence length86-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA712250.21875480373724424No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA600210.1843437286783171No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC362800.11142750831291288No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG356680.10954786015724854No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG334090.10260974711207571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC78150.025.2430465
GCGTAGC86550.022.9575882
TATTAGA158350.022.8553242
GTATTAG203950.022.7788851
GTGTTAT336150.021.9129181
TAGATAG45750.021.8185585
GCCGTCT309200.021.69469894-95
TGTTATG339300.021.1517242
TGTGCGT148300.020.7049548
GTTATGC355250.019.8420353
TTAGAAC96550.019.060373
TTATGCC379600.018.6066274
CGTAGCC121800.018.2162743
TAGGGTC197650.017.9992794
CATAGGG221650.017.3526922
TGCCGTC486150.017.0549794-95
GTGTGCG186600.016.9609897
CGCGTGT87500.016.7068711
CCATAGG222100.016.5507321
TATGCCC433900.016.190925
Produced by FastQC (version 0.11.5)