FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32559285
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA695850.2137178380913463No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA582170.1788030664678294No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAG369270.11341465268662994No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC366320.11250861313447148No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG330050.10136893362369598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA153950.026.6079772
GTATTAG203250.025.4459511
GTGTTAT342250.021.9413091
TGTGCGT128300.021.6411538
TGTTATG344800.021.2636622
TAGCCCC76050.020.8360425
GTTATGC362500.019.7815723
GCGTAGC78700.019.713912
CGGTTCG36950.019.586019
TTATGCC385500.018.7608594
TAGGGTC204800.018.684794
TTAGAAC93350.018.596083
TAGATAG44350.017.9178265
CGCGTGT64500.017.753531
CATAGGG226900.017.5946882
ACACCGT77600.017.5549836
CCATAGG228350.017.219841
TATGCCC420800.017.0971285
GAGTAGG96750.017.0688231
GTGTGCG169850.016.9598277
Produced by FastQC (version 0.11.5)