FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17051657
Sequences flagged as poor quality0
Sequence length87-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA203220.11917903345111855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC46700.027.664343
GCCGTCT567700.024.23905294-95
TAGATAG17050.024.1473465
CTTAGAA53150.024.0407352
TAGAACT65750.023.0318454
CGCGTGT27550.022.846441
AATTTAA87150.022.4269332
TGTGCGT62450.022.2808328
ATTTAAG87550.022.1616573
TATTAGA49300.021.790452
TAGGCCC24000.021.492644
TAAGCAT89650.021.3266
GAATTTA96550.020.6846731
GTATTAG84400.020.6344991
AAGCATA95050.020.0149637
TGCCGTC709800.019.81824994-95
ATATTAG106250.019.3307761
TAGGGTC64950.018.871014
CATAGGG69900.018.5507142
TCTCGAC54600.018.4612663
Produced by FastQC (version 0.11.5)