FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39396569
Sequences flagged as poor quality0
Sequence length86-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA561570.14254286965953814No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG402740.10222717617871749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT703050.023.99951294-95
TAGCCCC102950.023.967895
CGCGTGT77300.023.4830191
TGTGCGT167750.022.8547258
TATTAGA160550.022.5243782
GTATTAG208250.021.9277631
GCGTAGC114200.021.6903632
CGACCCG173100.019.798245
TGCCGTC909050.019.13805294-95
GTGTGCG209000.018.885487
TAGATAG46500.018.8063475
CGTAGCC153450.018.2672633
TAGGGTC234750.017.9011884
CGGGACG107350.017.173085
CATAGGG261400.017.0713582
CCGCGCG162500.017.047729
GTGCGTC246850.016.8527819
CCGTCTT631850.016.35631294-95
GTATGAA370950.016.311611
CCATAGG269650.016.0932771
Produced by FastQC (version 0.11.5)