FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7073494
Sequences flagged as poor quality0
Sequence length89-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA163800.23156872685549745No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT130060.18386952756304029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13350.021.2692381
TAGCCCC15850.021.1890495
GAGCGTA11350.019.5877426
CTACACT31300.018.588454
TATAGTG8900.018.0705475
CTAGTAC13350.017.716223
CGGTGGT81000.017.44060194-95
GCTTAGA16350.017.3666271
GCGTAGC17650.016.616112
GGGTATA15500.016.4870911
GTATTAG21450.016.3262481
TAAGAGT10900.016.056754
TGTGCGT23250.015.6657138
GCTTAGG16400.015.5751522
GTGCTAA20200.015.4623161
TACTAGG17250.015.3561132
TACACTA11400.015.3525055
TAGTACT23600.015.2329494
GTATTGG32250.015.114351
TCGGTGG114100.015.06499394-95
Produced by FastQC (version 0.11.5)