FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11160530
Sequences flagged as poor quality0
Sequence length43-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA468190.4195051668693154No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT393100.3522234159130435No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA155610.13942886224937345No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG121540.1089016381838497No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG114420.10252201284347608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG51600.023.7566261
TATTAGA49900.022.7515952
CGCGTGT22400.020.28421
TAATACT44900.018.3317554
CGGTGGT196100.018.1011994-95
TGTGCGT42750.017.9258638
GTATGAA100700.017.8133031
TATGAAT98450.017.4418322
TCGGTGG239600.017.4243194-95
TAGGGTC67550.017.297084
CATAGGG76700.016.9604932
ATAGGGT72800.016.5045413
CCATAGG78000.016.2620351
ATTAGAG76650.015.4286883
GTGCGTC52150.015.4204049
CTAATAC49550.015.0839343
TTAGAGG86950.014.9611254
GTTTAGG51000.014.9413691
CTATAGT9900.014.8124764
TTAGGAC59500.014.6285743
Produced by FastQC (version 0.11.5)