FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30820128
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384620.12479506898868167No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA366390.11888010328834454No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT57350.027.9239081
TTAGAAC81000.026.7733523
TGTGCGT140050.024.9514758
CTTAGAA89250.023.7680452
TAGATAG44450.023.542015
TAGAACT102950.022.4448384
GTGTGCG167900.021.2075337
TATTAGA104300.021.1555982
CGGGACG80900.020.9535455
GTATTAG145850.020.7905731
TAGCCCC62150.020.1896025
CGGTTCG41900.019.7763884
AGGACGC141600.019.4617869
TAGGGTC118750.019.219254
CATAGGG134100.018.9966582
CGGTGGT436300.018.1300394-95
CGTGTGG201150.017.7836071
CCATAGG137450.017.6489221
TAAGGTA87050.017.6237895
ATAGGGT134300.017.593283
Produced by FastQC (version 0.11.5)