FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15867329
Sequences flagged as poor quality0
Sequence length88-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168630.10627497545428093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG17650.023.3233995
GTATTAG49800.022.2464731
GTGTTAT77700.018.767411
TATTAGA36350.018.4843982
TGTTATG78400.017.7440472
GTTATGC74250.017.0787333
GTATTGG88150.017.0259591
CTATAGT16900.016.5189574
TAGGCCC25450.016.3610884
TAGCCCC29000.015.9898875
CGGTGGT184800.015.93669494-95
TCGGTGG239950.015.87539294-95
CGCGTGT39050.015.276511
TGTGCGT59100.015.2118988
ATATTAG49100.015.1388481
CTACACT56700.015.0212724
TTATGCC84950.014.9832554
TATACTG26300.014.9327455
TAATACT38650.014.8133474
TCTATAG20000.014.4316873
Produced by FastQC (version 0.11.5)