FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13538150
Sequences flagged as poor quality0
Sequence length86-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA217470.16063494642916498No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT174250.12871034816426175No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT35600.024.3207321
GTATTAG29350.022.8905261
GCCGTCT422200.022.83467394-95
TAGATAG11850.020.7608975
TGCCGTC531850.018.33781694-95
TGTGCGT64600.017.8718
GTATTGG55800.017.0426621
CCGTCTT325150.016.66503194-95
TATTAGA23100.015.5660042
TATTAGG15950.015.1281212
GTGCGTC81700.015.0574879
GTACTAA10100.014.9900851
TTAGGCA42850.014.9054144
GTGTGCG80350.014.7791327
ATGCCGT555550.014.65424992-93
CTACACT43800.014.150064
GCTTAGG25500.014.1009692
TAGACAG44650.014.0926045
TAGCCCC26900.013.8942765
TATGCCG593650.013.62514492-93
Produced by FastQC (version 0.11.5)