FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13538150
Sequences flagged as poor quality0
Sequence length88-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA214750.15862580928708872No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT180150.13306840299450073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG29300.022.4616721
TAGATAG11250.021.4484775
CGCGTGT32350.017.8558461
TGTGCGT56250.017.1587818
GAGTAGG47750.016.5591471
GCTTAGG28850.016.3995882
TATTAGA23600.016.238722
TATTAGG15300.015.1524712
GTACTAA10950.014.7014341
GTATTGG56650.014.5426721
CGGTGGT172700.014.28857994-95
GTGCGTC73550.014.216369
GTGTGCG72950.013.6847217
TAGGCAG49150.013.6692155
TTAGGCA46100.013.6499014
TAGGCCC21950.013.5795324
CTACACT44950.013.4728374
GTATGAA46850.013.3401051
TCGGTGG212700.013.28384494-95
GTCCTAG18350.013.1591851
Produced by FastQC (version 0.11.5)