FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11389797
Sequences flagged as poor quality0
Sequence length35-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA296470.2602943669672076No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT240080.2107851439318892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123260.10821966361648061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT29100.026.0457381
TAGATAG11500.024.6947425
GTATTAG23800.022.093051
TGTGCGT59300.020.7524668
TAGCCCC24050.020.2709085
TATTAGC11550.018.8506762
CTATAGT10650.018.6659774
TATAGTG12150.017.5302185
TAGGCCC19750.017.0154084
GTGCGTC75550.016.8526769
GTGTGCG73600.016.5274727
GTATAAT8650.016.4291381
GTGTTAT41100.016.1361
GCGTAGC31250.015.9034132
ATATTAG33950.015.76697351
TGTTATG39950.015.7574492
GTTATGC37650.015.4629093
TATCTAG24550.015.4364751
AATACGG18150.015.3860255
TATTAGT35850.015.0510482
Produced by FastQC (version 0.11.5)