FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27630896
Sequences flagged as poor quality0
Sequence length86-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT64900.023.3296761
GCCGTCT913100.022.24225494-95
GTATTAG87300.021.950491
CGACCCG134100.021.7690475
TAGATAG26300.021.5878055
TATTAGA66700.020.9266072
CGGGACG111950.020.9201165
TAGCCCC69300.020.6867565
GTGTTAT140500.020.4079691
TGTTATG144550.019.377882
TGTGCGT124150.019.1326268
GTTATGC145750.018.9577713
ACCCGCG134750.018.3636537
TGCCGTC1101450.018.15263294-95
GCGTAGC84100.017.4971372
CCGCGCG179600.016.9668129
CCGTCTT723400.016.78237394-95
TTATGCC163300.016.7465234
CTATAGT26900.016.3573784
TATAGTG30900.016.2304445
Produced by FastQC (version 0.11.5)